Bioprocesses operated in batch mode can induce adverse effects like overflow metabolism, substrate inhibition, osmotic inhibition, oxygen limitation and catabolite repression. (…)
Authors: Tobias Habicher, Vroni Czotscher, Tobias Klein, Andreas Daub, Timm Keil, Jochen Büchs
Journal: Biotechnology Bioengineering
The use of benchtop bioreactors (BRs) for the development of mammalian cell perfusion cultures is expensive and time consuming, given its complexity in equipment and operation (…)
Authors: Wolf, Moritz K. F.; Lorenz, Veronika; Karst, Daniel J.; Souquet, Jonathan; ] ; Karst, DJ (Karst, Daniel J.)[ 1 ] ; Souquet, J (Souquet, Jonathan)[ 2 ] ; Broly, H (Broly, Herve; Morbidelli, Massimo
Journal: Biotechnology and Bioengineering, Volume: 115, Issue: 11, Pages: 2703-2713
The cell‐specific growth rate (µ) is a critical process parameter for antibody production processes performed by animal cell cultures, as it describes the cell growth and refl (…)
Authors: Li, Meng-Yao; Ebel, Bruno; Blanchard, Fabrice; Paris, Cedric; Guedon, Emmanuel; Marc, Annie
Journal: Biotechnology and Bioengineering, Volume: 116 Issue: 5, Pages: 985-993
This chapter about single‐use bioreactors (SUBs) aims to provide an overview of the advances in this – compared with conventional bioreactors – young field of bioreactor devel (…)
Authors: Nico M.G. Oosterhuis; Stefan Junne
Journal: Bioreactors: Design, Operation and Novel Applications
The Gram-negative bacterium E. coli is the host of choice for the production of a multitude of recombinant proteins in industry. Generally, cultivation is easy, media are chea (…)
Authors: Slouka, Christoph; Kopp, Julian; Strohmer, Daniel; Kager, Julian; Spadiut, Oliver; Herwig, Christoph
Journal: Journal of Biotechnology, Volume: 296, Pages: 75-82
To overcome catabolite repression, industrial fermentation processes are usually operated in substrate‐limited fed‐batch mode. Therefore, the implementation of such an operati (…)
Authors: Tobias Habicher; Arian John; Niklas Scholl; Andreas Daub; Tobias Klein; Priya Philip; Jochen Büchs
Journal: Biotechnology and Bioengineering
The application of standard sensor electrodes is often limited to stirred tank reactors due to their wired nature and their large space requirement. For other cultivation syst (…)
Authors: Tim Lauterbach; Tobias Lüke; Maik-Julian Büker; Christian Hedayat; Tassilo Gernandt; Rüdiger Moll; Michael Grösel; Stephan Lenk; Franziska Seidel; Dietmar Brunner; Thomas Bley; Thomas Walther; Felix Lenk
Journal: Sensors and Actuators A: Physica, Volume: 287
Microtiter plates with 96 wells are routinely used in biofilm research mainly because they enable high-throughput assays. These platforms are used in a variety of conditions r (…)
Authors: Gomes, L. C.; Moreira, J. M. R.; Teodosio, J. S.; Araujo, J. D. P.; Miranda, J. M.; Simoes, M.; Melo, L. F.; Mergulhao, F. J.
Journal: Biofouling, Volume: 30, Issue: 5, Pages: 535-546
Microtiter plates are one of the most widely used platforms for biofilm studies. However, these systems have been used rather blindly without knowledge of the flow characteris (…)
Authors: Moreira, Joana M. R.; Gomes, Luciana C.; Araujo, Jose D. P.; Miranda, Joao M.; Simoes, Manuel; Melo, Luis F.; Mergulhao, Filipe J.
Journal: Chemical Engineering Science, Volume: 94, Pages: 192-199
Tissue engineering is a promising approach for bone regeneration; yet challenges remain that limit successful translation to patients. It is necessary to understand how real-w (…)
Authors: De Silva Thompson, David; Peticone, Carlotta; Burova, Iva; Shipley, Rebecca J.Knowles, Jonathan C.; Kim, Hae-Won; Micheletti, Martina; Wall, Ivan B.
Journal: Journal of Tissue Engineering, Volume: 10
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