FAQs

E. coli is a very respiratory active microorganism so the filling volume should not exceed 5 - 10 % of the total flask volume (i. e. 10 to maximum 25 mL in a 250 mL flask). Recommended culture conditions for the aerobic cultivation of E. coli in a 250 mL wide neck flask with cotton plug are as follows:
10 mL filling volume
300 rpm shaking speed
50 mm shaking diameter