We use cookies in order to optimize our website for you. If you continue reading on this website, you agree to the use of cookies.
You can find more information on the page about Cookies.
We provide a summary of references from literature and Dr. Shaker's recommendations.
Dr. Shaker`s Recommendation
| Mammalian Cell culture | Insect Cells | Plant Cell culture | ||
|---|---|---|---|---|
| TubeSpin 50 | CHO | HEK-293 | SF-9 | N. tabacum BY-2 |
| Shaking Speed [rpm] | 180 - 250 | 180 - 250 | 180 - 250 | 180 - 250 |
| Throw [mm] | 50 | 50 | 50 | 50 |
| Working Volume [mL] | 15 - 30 | 15 - 30 | 5 - 10 | 5 - 10 |
| TubeSpin 600 | CHO | HEK-293 | SF-9 | N. tabacum BY-2 |
| Shaking Speed [rpm] | 180 - 250 | 180 - 250 | 180 - 250 | 180 - 250 |
| Throw [mm] | 50 | 50 | 50 | 50 |
| Working Volume [mL] | 300 | 300 | 200 | 150 |
Methods from Literature
| TubeSpin 50 | CHO | CHO | CHO | CHO | SF-9 |
|---|---|---|---|---|---|
| Shaking Speed [rpm] | 180-300 | 160-200 | 140 | 200 | 160-200 |
| Throw [mm] | 50 | n.s. | 50 | 25 | n.s. |
| Working Volume [mL] | 14-34 | 10-35 | 5-10 | 5 | 10-35 |
| Reference | Strnad (2010), Biotechnol. Prog.: Vol. 26 (3); pp 653-663 | Xie (2011), Cytotechnology: Vol. 63 (4); pp 345-350 | Stettler (2007), Biotechnol. Prog.: Vol. 23 (6); pp 1340 -1346 | De Jesus (2004), Biochem. Eng. Journal: Vol 17, No. 3; pp 217 - 223 | Shen (2011), BMC Proc.: 5 (Suppl 8); P37 |
| Tube Spin 600 | CHO | Crypthecodinium cohnii (marine microalgae) | |||
| Shaking Speed [rpm] | 120-220 | 230 | |||
| Throw [mm] | 50 | 25 | |||
| Working Volume [mL] | 300 | 100 | |||
| Reference | Monteil (2013), Biochem. Eng. Journal; Vol. 76, pp 6–12 | Hillig (2014), Adv Biochem Eng Biotechnol; Vol. 138: pp 179-206 |
We use cookies in order to optimize our website for you. If you continue reading on this website, you agree to the use of cookies.
You can find more information on the page about Cookies.