We are not aware of any situation where microcarriers should not be used with the TPP bioreactor. Variations of microcarrier culture performance of CHO cells in different vessels (50 mL vented tubes and 250 mL shake flasks) were investigated in the following publication:
Evaluation of macroporous and microporous carriers for CHO-K1 cell growth and monoclonal antibody production.
Rodrigues ME, Costa AR, Fernandes P, Henriques M, Cunnah P, Melton DW, Azeredo J, Oliveira R. J Microbiol Biotechnol. (2013) Sep 28; Vol. 23(9): pp 1308-1321
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